› 3ra4

X-ray diffraction
2.7Å resolution

Structural studies of AAV8 capsid transitions associated with endosomal trafficking

Released:
DOI: 10.2210/pdb3ra4/pdb
PDB entry 3ra4 coloured by chain, front view.
PDB entry 3ra4 coloured by chain, side view.
PDB entry 3ra4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural studies of adeno-associated virus serotype 8 capsid transitions associated with endosomal trafficking.
Nam HJ, Gurda BL, McKenna R, Potter M, Byrne B, Salganik M, Muzyczka N, Agbandje-McKenna M
J Virol 85 11791-9 (2011)
PMID: 21900159

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 120-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116797 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Capsid protein Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58.53 KDa
Source organism: Adeno-associated virus - 8
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8JQF8 (Residues: 220-738; Coverage: 70%)
Sequence domains: Parvovirus coat protein VP2
Structure domains: Parvovirus coat protein VP1/VP2
DNA (5'-D(*CP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 2 nucleotides
Theoretical weight: 557 Da
Source organism: Aequorea victoria
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6322
Unit cell:
a: 257.94Å b: 257.94Å c: 448.69Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.213
Expression system: Homo sapiens

Quick links

Citations

15 review citations

Intracellular transport of recombinant adeno-associated virus vectors.
Nonnenmacher et al. (2012)
14 more

3 mentions without citation

Structural studies of adeno-associated virus serotype 8 capsid transitions associated with endosomal trafficking.
Nam et al. (2011)
2 more