› 3rbh

X-ray diffraction
2.3Å resolution

Structure of alginate export protein AlgE from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3rbh/pdb
PDB entry 3rbh coloured by chain, front view.
PDB entry 3rbh coloured by chain, side view.
PDB entry 3rbh coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis for alginate secretion across the bacterial outer membrane.
Whitney JC, Hay ID, Li C, Eckford PD, Robinson H, Amaya MF, Wood LF, Ohman DE, Bear CE, Rehm BH, Howell PL
Proc Natl Acad Sci U S A 108 13083-8 (2011)
PMID: 21778407

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate production protein AlgE Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 479 amino acids
Theoretical weight: 53.78 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P18895 (Residues: 33-490; Coverage: 100%)
Gene names: PA3544, alg76, algE
Sequence domains: Alginate export
Structure domains: Porin

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand EDO 24 x EDO
Ligand C8E 29 x C8E
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 162.018Å b: 90.696Å c: 160.279Å
α: 90° β: 107.65° γ: 90°
R-values:
R R work R free
0.223 0.221 0.259
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions.
Römling et al. (2015)
11 more

11 mentions without citation

Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.
Whitney et al. (2013)
10 more