› 3rbx

X-ray diffraction
2.8Å resolution

MthK RCK domain D184N mutant, Ca2+-bound

Released:
DOI: 10.2210/pdb3rbx/pdb
PDB entry 3rbx coloured by chain, front view.
PDB entry 3rbx coloured by chain, side view.
PDB entry 3rbx coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Primary publication:
Structures of multiple Ca2+-binding sites in a K+ channel RCK domain
Pau VP, Smith FJ, Taylor AB, Samakai E, Callaghan MM, Abarca-Heidemann K, Parfenova LV, Hart PJ, Rothberg BS
Proc. Natl. Acad. Sci. U.S.A. (2011)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 234 amino acids
Theoretical weight: 25.91 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O27564 (Residues: 107-336; Coverage: 69%)
Gene names: MTH_1520, mthK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 12 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6522
Unit cell:
a: 119.12Å b: 119.12Å c: 351.052Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.24 0.274
Expression system: Escherichia coli

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