› 3rdy

X-ray diffraction
1.84Å resolution

Crystal Structure of buckwheat trypsin inhibitor rBTI at 1.84 angstrom resolution

Released:
DOI: 10.2210/pdb3rdy/pdb
Model geometry
Fit model/data
PDB entry 3rdy coloured by chain, front view.
PDB entry 3rdy coloured by chain, side view.
PDB entry 3rdy coloured by chain, top view.
Source organism: Fagopyrum esculentum
Primary publication:
Conformational changes of rBTI from buckwheat upon binding to trypsin: implications for the role of the P(8)' residue in the potato inhibitor I family.
Wang L, Zhao F, Li M, Zhang H, Gao Y, Cao P, Pan X, Wang Z, Chang W
OpenAccess logo PLoS One 6 e20950 (2011)
PMID: 21698291

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase inhibitor Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 9.02 KDa
Source organism: Fagopyrum esculentum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S9F3 (Residues: 1-69; Coverage: 100%)
Sequence domains: Potato inhibitor I family
Structure domains: Trypsin Inhibitor V, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BSRF BEAMLINE 1W2B
Spacegroup: P43212
Unit cell:
a: 62.654Å b: 62.654Å c: 45.906Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.19 0.216
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure, dynamics, assembly, and evolution of protein complexes.
Marsh et al. (2015)
4 other articles

2 mentions without citation

Conformational changes of rBTI from buckwheat upon binding to trypsin: implications for the role of the P(8)' residue in the potato inhibitor I family.
Wang et al. (2011)
1 more