› 3red

X-ray diffraction
3.03Å resolution

3.0 A structure of the Prunus mume hydroxynitrile lyase isozyme-1

Released:
DOI: 10.2210/pdb3red/pdb
PDB entry 3red coloured by chain, front view.
PDB entry 3red coloured by chain, side view.
PDB entry 3red coloured by chain, top view.
Source organism: Prunus mume
Entry authors: Cielo CBC, Yamane T, Asano Y, Watanabe N, Suzuki A, Fukuta Y

Function and Biology Details

Reaction catalysed: 
(R)-mandelonitrile = cyanide + benzaldehyde
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110925 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-mandelonitrile lyase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 521 amino acids
Theoretical weight: 56.41 KDa
Source organism: Prunus mume
UniProt:
  • Canonical: B9X0I1 (Residues: 29-549; Coverage: 95%)
Gene name: PmHNL
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 12 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P1
Unit cell:
a: 112.019Å b: 113.302Å c: 169.488Å
α: 89.92° β: 79.13° γ: 80.83°
R-values:
R R work R free
0.194 0.191 0.253

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