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X-ray diffraction
1.76Å resolution

Crystal structure of mitoNEET

Released:
DOI: 10.2210/pdb3ree/pdb
PDB entry 3ree coloured by chain, front view.
PDB entry 3ree coloured by chain, side view.
PDB entry 3ree coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Complexes of the outer mitochondrial membrane protein mitoNEET with resveratrol-3-sulfate.
Arif W, Xu S, Isailovic D, Geldenhuys WJ, Carroll RT, Funk MO
Biochemistry 50 5806-11 (2011)
PMID: 21591687

Function and Biology Details

Reaction catalysed: 
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDGSH iron-sulfur domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 9.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZ45 (Residues: 32-108; Coverage: 71%)
Gene names: C10orf70, CISD1, MDS029, ZCD1
Sequence domains: Iron-binding zinc finger CDGSH type
Structure domains: CDGSH iron-sulfur domain, mitoNEET-type

Ligands and Environments

1 bound ligand:
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I4122
Unit cell:
a: 58.64Å b: 58.64Å c: 177.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

mitoNEET as a novel drug target for mitochondrial dysfunction.
Geldenhuys et al. (2014)
1 more

4 mentions without citation

Complexes of the outer mitochondrial membrane protein mitoNEET with resveratrol-3-sulfate.
Arif et al. (2011)
3 more