› 3ref

X-ray diffraction
1.95Å resolution

Crystal structure of EhRho1 bound to GDP and Magnesium

Released:
DOI: 10.2210/pdb3ref/pdb
PDB entry 3ref coloured by chain, front view.
PDB entry 3ref coloured by chain, side view.
PDB entry 3ref coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Unique structural and nucleotide exchange features of the Rho1 GTPase of Entamoeba histolytica.
Bosch DE, Wittchen ES, Qiu C, Burridge K, Siderovski DP
J Biol Chem 286 39236-46 (2011)
PMID: 21930699

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-like GTP-binding protein RHO1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 21.43 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31021 (Residues: 1-191; Coverage: 89%)
Gene names: EHI_029020, RHO1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 50.3Å b: 54.476Å c: 132.285Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.156 0.197
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

G protein signaling in the parasite Entamoeba histolytica.
Bosch et al. (2013)
1 more

2 mentions without citation

Entamoeba histolytica Rho1 regulates actin polymerization through a divergent, diaphanous-related formin.
Bosch et al. (2012)
1 more