3reg

X-ray diffraction
1.8Å resolution

Crystal structure of EhRho1 bound to a GTP analog and Magnesium

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-151892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-like GTP-binding protein RHO1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 21.43 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31021 (Residues: 1-191; Coverage: 89%)
Gene names: EHI_029020, RHO1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 36.468Å b: 39.497Å c: 63.578Å
α: 81.75° β: 80.76° γ: 65.44°
R-values:
R R work R free
0.174 0.172 0.222
Expression system: Escherichia coli BL21