› 3ren

X-ray diffraction
2Å resolution

CPF_2247, a novel alpha-amylase from Clostridium perfringens

Released:
DOI: 10.2210/pdb3ren/pdb
PDB entry 3ren coloured by chain, front view.
PDB entry 3ren coloured by chain, side view.
PDB entry 3ren coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Structural analysis of CPF_2247, a novel α-amylase from Clostridium perfringens.
Ficko-Blean E, Stuart CP, Boraston AB
Proteins 79 2771-7 (2011)
PMID: 21905105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase, family 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 40.57 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2YP60 (Residues: 43-371; Coverage: 89%)
Gene name: CPF_2247
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand SO4 3 x SO4
Ligand EDO 13 x EDO
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 65.35Å b: 75.06Å c: 158.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.224
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The successful experimental induction of necrotic enteritis in chickens by Clostridium perfringens: a critical review.
Shojadoost et al. (2012)
2 more

4 mentions without citation

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
3 more