› 3reo

X-ray diffraction
1.9Å resolution

Monolignol O-methyltransferase (MOMT)

Released:
DOI: 10.2210/pdb3reo/pdb
PDB entry 3reo coloured by chain, front view.
PDB entry 3reo coloured by chain, side view.
PDB entry 3reo coloured by chain, top view.
Source organism: Clarkia breweri
Entry authors: Bhuiya MW, Liu CJ

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + isoeugenol = S-adenosyl-L-homocysteine + isomethyleugenol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(Iso)eugenol O-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 368 amino acids
Theoretical weight: 39.99 KDa
Source organism: Clarkia breweri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O04385 (Residues: 1-368; Coverage: 100%)
Gene name: IEMT1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
1 bound ligand:
Ligand EUG 3 x EUG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 66.241Å b: 150.754Å c: 68.302Å
α: 90° β: 92.67° γ: 90°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Escherichia coli BL21(DE3)

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