3rfz

X-ray diffraction
2.8Å resolution

Crystal structure of the FimD usher bound to its cognate FimC:FimH substrate

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-140005 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Type 1 fimbrin D-mannose specific adhesin Chains: A, D
Molecule details ›
Chains: A, D
Length: 279 amino acids
Theoretical weight: 29.18 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08191 (Residues: 22-300; Coverage: 100%)
Gene names: JW4283, b4320, fimH
Sequence domains:
Structure domains: Fimbrial-type adhesion domain
Outer membrane usher protein FimD Chains: B, E
Molecule details ›
Chains: B, E
Length: 843 amino acids
Theoretical weight: 92.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P30130 (Residues: 46-878; Coverage: 100%)
Gene names: JW5780, b4317, fimD
Sequence domains:
Structure domains:
Chaperone protein FimC Chains: C, F
Molecule details ›
Chains: C, F
Length: 211 amino acids
Theoretical weight: 23.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P31697 (Residues: 37-241; Coverage: 100%)
Gene names: JW4279, b4316, fimC
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 126.19Å b: 95.9Å c: 144.57Å
α: 90° β: 112.13° γ: 90°
R-values:
R R work R free
0.226 0.223 0.282
Expression system: Escherichia coli