› 3rhw

X-ray diffraction
3.26Å resolution

C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab and ivermectin

Released:
DOI: 10.2210/pdb3rhw/pdb
PDB entry 3rhw coloured by chain, front view.
PDB entry 3rhw coloured by chain, side view.
PDB entry 3rhw coloured by chain, top view.
Source organisms:
Primary publication:
Principles of activation and permeation in an anion-selective Cys-loop receptor.
Hibbs RE, Gouaux E
Nature 474 54-60 (2011)
PMID: 21572436

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-211640 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Avermectin-sensitive glutamate-gated chloride channel GluCl alpha Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 347 amino acids
Theoretical weight: 39.64 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
Structure domains:
Mouse monoclonal Fab fragment, heavy chain Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 221 amino acids
Theoretical weight: 23.92 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Mouse monoclonal Fab fragment, light chain Chains: K, L, M, N, O
Molecule details ›
Chains: K, L, M, N, O
Length: 210 amino acids
Theoretical weight: 22.62 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

6 bound ligands:
Ligand IVM 5 x IVM
Ligand LMT 3 x LMT
Ligand NAG 3 x NAG
Ligand OCT 3 x OCT
Ligand UND 1 x UND
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 154.85Å b: 154.85Å c: 574.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.27
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

122 review citations

Structure, function, and modulation of GABA(A) receptors.
Sigel et al. (2012)
121 more

84 mentions without citation

P2X receptors as drug targets.
North et al. (2013)
83 more