› 3ric

X-ray diffraction
2.1Å resolution

Crystal Structure of D48V||A47D mutant of Human Glycolipid Transfer Protein complexed with 3-O-sulfo-galactosylceramide containing nervonoyl acyl chain (24:1)

Released:
DOI: 10.2210/pdb3ric/pdb
PDB entry 3ric coloured by chain, front view.
PDB entry 3ric coloured by chain, side view.
PDB entry 3ric coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Enhanced selectivity for sulfatide by engineered human glycolipid transfer protein.
Samygina VR, Popov AN, Cabo-Bilbao A, Ochoa-Lizarralde B, Goni-de-Cerio F, Zhai X, Molotkovsky JG, Patel DJ, Brown RE, Malinina L
Structure 19 1644-54 (2011)
PMID: 22078563

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycolipid transfer protein Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZD2 (Residues: 1-209; Coverage: 100%)
Gene name: GLTP
Sequence domains: Glycolipid transfer protein (GLTP)
Structure domains: Glycolipid transfer protein

Ligands and Environments

1 bound ligand:
Ligand CIS 1 x CIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 76.871Å b: 48.128Å c: 69.086Å
α: 90° β: 123.82° γ: 90°
R-values:
R R work R free
0.184 0.182 0.219
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Sphingolipids and their metabolism in physiology and disease.
Hannun et al. (2018)
6 more

2 mentions without citation

Enhanced selectivity for sulfatide by engineered human glycolipid transfer protein.
Samygina et al. (2011)
1 more