› 3rii

X-ray diffraction
2Å resolution

Crystal structure of the catalytic domain of UCHL5, a proteasome-associated human deubiquitinating enzyme, reveals an unproductive form of the enzyme

Released:
DOI: 10.2210/pdb3rii/pdb
PDB entry 3rii coloured by chain, front view.
PDB entry 3rii coloured by chain, side view.
PDB entry 3rii coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic domain of UCHL5, a proteasome-associated human deubiquitinating enzyme, reveals an unproductive form of the enzyme.
Maiti TK, Permaul M, Boudreaux DA, Mahanic C, Mauney S, Das C
FEBS J 278 4917-26 (2011)
PMID: 21995438

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5K5 (Residues: 1-228; Coverage: 69%)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains: Ubiquitin carboxyl-terminal hydrolase, family 1
Structure domains: Peptidase C12, ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 45.395Å b: 99.034Å c: 48.145Å
α: 90° β: 96.39° γ: 90°
R-values:
R R work R free
0.177 0.175 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Substrate specificity of the ubiquitin and Ubl proteases.
Ronau et al. (2016)
4 more

4 mentions without citation

Crystal structure of the catalytic domain of UCHL5, a proteasome-associated human deubiquitinating enzyme, reveals an unproductive form of the enzyme.
Maiti et al. (2011)
3 more