› 3riq

X-ray diffraction
1.5Å resolution

Siphovirus 9NA tailspike receptor binding domain

Released:
DOI: 10.2210/pdb3riq/pdb
PDB entry 3riq coloured by chain, front view.
PDB entry 3riq coloured by chain, side view.
PDB entry 3riq coloured by chain, top view.
Source organism: Siphovirus 9NA
Primary publication:
Tail morphology controls DNA release in two Salmonella phages with one lipopolysaccharide receptor recognition system.
Andres D, Roske Y, Doering C, Heinemann U, Seckler R, Barbirz S
Mol Microbiol 83 1244-53 (2012)
PMID: 22364412

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tailspike protein Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 59.41 KDa
Source organism: Siphovirus 9NA
Expression system: Escherichia coli
UniProt:
  • Canonical: G8GV21 (Residues: 131-673; Coverage: 81%)
Gene name: 9NA_055
Sequence domains: Salmonella phage P22 tail-spike
Structure domains: Pectate Lyase C-like

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I213
Unit cell:
a: 149.132Å b: 149.132Å c: 149.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.128 0.17
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Targeting mechanisms of tailed bacteriophages.
Nobrega et al. (2018)
6 more

2 mentions without citation

Bacteriophage tailspike protein based assay to monitor phase variable glucosylations in Salmonella O-antigens.
Schmidt et al. (2016)
1 more