3rkk

X-ray diffraction
2.35Å resolution

Crystal Structure of New Delhi Metallo-Beta-Lactamase-1 from Klebsiella pneumoniae

Released:

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-111680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 25.1 KDa
Source organism: Klebsiella pneumoniae
Expression system: Cloning vector pNF2214
UniProt:
  • Canonical: C7C422 (Residues: 39-270; Coverage: 96%)
Gene name: blaNDM-1
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 59.76Å b: 50.862Å c: 70.717Å
α: 90° β: 106.98° γ: 90°
R-values:
R R work R free
0.212 0.208 0.283
Expression system: Cloning vector pNF2214