› 3rlb

X-ray diffraction
2Å resolution

Crystal structure at 2.0 A of the S-component for thiamin from an ECF-type ABC transporter

Released:
DOI: 10.2210/pdb3rlb/pdb
PDB entry 3rlb coloured by chain, front view.
PDB entry 3rlb coloured by chain, side view.
PDB entry 3rlb coloured by chain, top view.
Source organism: Lactococcus lactis subsp. cremoris NZ9000
Primary publication:
The structural basis of modularity in ECF-type ABC transporters.
Erkens GB, Berntsson RP, Fulyani F, Majsnerowska M, Vujičić-Žagar A, Ter Beek J, Poolman B, Slotboom DJ
Nat Struct Mol Biol 18 755-60 (2011)
PMID: 21706007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ThiT Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.23 KDa
Source organism: Lactococcus lactis subsp. cremoris NZ9000
Expression system: Lactococcus lactis subsp. cremoris NZ9000
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

2 bound ligands:
Ligand BNG 11 x BNG
Ligand VIB 2 x VIB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 61.654Å b: 84.654Å c: 127.301Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.207 0.206 0.23
Expression system: Lactococcus lactis subsp. cremoris NZ9000

Quick links

Citations

11 review citations

Structure and mechanism of ABC transporters.
Wilkens S. (2015)
10 more

9 mentions without citation

Structural diversity of ABC transporters.
ter Beek et al. (2014)
8 more