› 3rli

X-ray diffraction
1.85Å resolution

Crystal structure of monoacylglycerol lipase from Bacillus sp. H257 in complex with PMSF

Released:
DOI: 10.2210/pdb3rli/pdb
PDB entry 3rli coloured by chain, front view.
PDB entry 3rli coloured by chain, side view.
PDB entry 3rli coloured by chain, top view.
Source organism: Bacillus sp. H-257
Primary publication:
The structure of monoacylglycerol lipase from Bacillus sp. H257 reveals unexpected conservation of the cap architecture between bacterial and human enzymes.
Rengachari S, Bezerra GA, Riegler-Berket L, Gruber CC, Sturm C, Taschler U, Boeszoermenyi A, Dreveny I, Zimmermann R, Gruber K, Oberer M
Biochim Biophys Acta 1821 1012-21 (2012)
PMID: 22561231

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermostable monoacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 29.56 KDa
Source organism: Bacillus sp. H-257
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P82597 (Residues: 1-250; Coverage: 100%)
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand MRD 1 x MRD
Ligand PMS 1 x PMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 38.05Å b: 70.69Å c: 43.44Å
α: 90° β: 111.65° γ: 90°
R-values:
R R work R free
0.158 0.156 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
1 more

3 mentions without citation

Changes in Membrane Protein Structural Biology.
Birch et al. (2020)
2 more