› 3rno

X-ray diffraction
2.5Å resolution

Crystal Structure of Mouse Apolipoprotein A-I Binding Protein in Complex with NADP.

Released:
DOI: 10.2210/pdb3rno/pdb
PDB entry 3rno coloured by chain, front view.
PDB entry 3rno coloured by chain, side view.
PDB entry 3rno coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Identification of unknown protein function using metabolite cocktail screening.
Shumilin IA, Cymborowski M, Chertihin O, Jha KN, Herr JC, Lesley SA, Joachimiak A, Minor W
Structure 20 1715-25 (2012)
PMID: 22940582

Function and Biology Details

Reaction catalysed: 
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-185092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H-hydrate epimerase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8K4Z3 (Residues: 25-282; Coverage: 92%)
Gene names: Aibp, Apoa1bp, Naxe
Sequence domains: YjeF-related protein N-terminus
Structure domains: YjeF N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand NAP 1 x NAP
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: R32
Unit cell:
a: 126.664Å b: 126.664Å c: 108.494Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.219
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Zebrafish models of dyslipidemia: relevance to atherosclerosis and angiogenesis.
Fang et al. (2014)
10 more

2 mentions without citation

A pathway for repair of NAD(P)H in plants.
Colinas et al. (2014)
1 more