› 3roo

X-ray diffraction
2Å resolution

Murine class I major histocompatibility complex H-2Kb in complex with immunodominant LCMV-derived gp34-41 peptide

Released:
DOI: 10.2210/pdb3roo/pdb
PDB entry 3roo coloured by chain, front view.
PDB entry 3roo coloured by chain, side view.
PDB entry 3roo coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Nitro-tyrosination of the immunodominant LCMV epitope gp34-41 alters both its capacity to stabilize H-2Kb and the molecular surface of the MHC complex, affecting TCR recognition
Madhurantakam C, Duru AD, Leong C, Sandalova T, Webb JR, Achour A
PLoS ONE (2012)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135045 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 275 amino acids
Theoretical weight: 31.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01901 (Residues: 22-296; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Stable signal peptide Chains: E, F
Molecule details ›
Chains: E, F
Length: 8 amino acids
Theoretical weight: 916 Da
UniProt:
  • Canonical: P07399 (Residues: 34-40; Coverage: 1%)
Gene names: GP-C, GPC

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand MPD 2 x MPD
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 88.448Å b: 92.588Å c: 128.827Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.267
Expression system: Escherichia coli BL21(DE3)

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