› 3rr1

X-ray diffraction
1.95Å resolution

Crystal structure of enolase PRK14017 (target EFI-500653) from Ralstonia pickettii 12J

Released:
DOI: 10.2210/pdb3rr1/pdb
PDB entry 3rr1 coloured by chain, front view.
PDB entry 3rr1 coloured by chain, side view.
PDB entry 3rr1 coloured by chain, top view.
Source organism: Ralstonia pickettii 12J
Entry authors: Patskovsky Y, Hillerich B, Seidel RD, Zencheck WD, Toro R, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactonate dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 45.07 KDa
Source organism: Ralstonia pickettii 12J
Expression system: Escherichia coli
UniProt:
  • Canonical: B2UCA8 (Residues: 2-382; Coverage: 100%)
Gene names: Rpic_2990, dgoD
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand MLT 1 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 118.011Å b: 118.011Å c: 114.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.174
Expression system: Escherichia coli

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