› 3rwo

X-ray diffraction
1.7Å resolution

Crystal structure of YPT32 in complex with GDP

Released:
DOI: 10.2210/pdb3rwo/pdb
PDB entry 3rwo coloured by chain, front view.
PDB entry 3rwo coloured by chain, side view.
PDB entry 3rwo coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The activation cycle of Rab GTPase Ypt32 reveals structural determinants of effector recruitment and GDI binding.
Sultana A, Jin Y, Dregger C, Franklin E, Weisman LS, Khan AR
FEBS Lett 585 3520-7 (2011)
PMID: 22024479

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-156445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein YPT32/YPT11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 20.6 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P51996 (Residues: 7-188; Coverage: 82%)
Gene names: YGL210W, YPT11, YPT32
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 130Å b: 45.2Å c: 73.4Å
α: 90° β: 110.5° γ: 90°
R-values:
R R work R free
0.169 0.167 0.203
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
1 more

4 mentions without citation

The crystal structure of a voltage-gated sodium channel.
Payandeh et al. (2011)
3 more