› 3ry0

X-ray diffraction
1.4Å resolution

Crystal structure of TomN, a 4-Oxalocrotonate Tautomerase homologue in Tomaymycin biosynthetic pathway

Released:
DOI: 10.2210/pdb3ry0/pdb
PDB entry 3ry0 coloured by chain, front view.
PDB entry 3ry0 coloured by chain, side view.
PDB entry 3ry0 coloured by chain, top view.
Source organism: Streptomyces achromogenes
Primary publication:
Kinetic, crystallographic, and mechanistic characterization of TomN: elucidation of a function for a 4-oxalocrotonate tautomerase homologue in the tomaymycin biosynthetic pathway.
Burks EA, Yan W, Johnson WH, Li W, Schroeder GK, Min C, Gerratana B, Zhang Y, Whitman CP
Biochemistry 50 7600-11 (2011)
PMID: 21809870

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111019 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-oxalocrotonate tautomerase-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 6.94 KDa
Source organism: Streptomyces achromogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: C0LTT5 (Residues: 2-66; Coverage: 99%)
Gene name: tomN
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I4132
Unit cell:
a: 117.637Å b: 117.637Å c: 117.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.251
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Identification and characterization of new family members in the tautomerase superfamily: analysis and implications.
Huddleston et al. (2014)
1 more

6 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
5 more