› 3ry6

X-ray diffraction
3.8Å resolution

Complex of fcgammariia (CD32) and the FC of human IGG1

Released:
DOI: 10.2210/pdb3ry6/pdb
PDB entry 3ry6 coloured by chain, front view.
PDB entry 3ry6 coloured by chain, side view.
PDB entry 3ry6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Fc gammaRIIa recognition of human IgG and formation of inflammatory signaling complexes.
Ramsland PA, Farrugia W, Bradford TM, Sardjono CT, Esparon S, Trist HM, Powell MS, Tan PS, Cendron AC, Wines BD, Scott AM, Hogarth PM
J Immunol 187 3208-17 (2011)
PMID: 21856937

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134856 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 24.24 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P01857 (Residues: 114-327; Coverage: 54%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor II-a Chain: C
Molecule details ›
Chain: C
Length: 167 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P12318 (Residues: 40-206; Coverage: 59%)
Gene names: CD32, FCG2, FCGR2A, FCGR2A1, IGFR2
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG, BMA, MAN, GAL, SIA, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, SIA, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, NDG, BMA, MAN, FUL
Carbohydrate polymer
2 bound ligands:
Ligand GOL 4 x GOL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 153.38Å b: 255.55Å c: 58.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.252 0.426
Expression systems:
  • Mus musculus
  • Spodoptera frugiperda

Quick links

Citations

25 review citations

IgG subclasses and allotypes: from structure to effector functions.
Vidarsson et al. (2014)
24 more

30 mentions without citation

IgG Fc engineering to modulate antibody effector functions.
Wang et al. (2018)
29 more