› 3ry9

X-ray diffraction
1.95Å resolution

Crystal Structure of the Resurrected Ancestral Glucocorticoid Receptor 1 in complex with DOC

Released:
DOI: 10.2210/pdb3ry9/pdb
PDB entry 3ry9 coloured by chain, front view.
PDB entry 3ry9 coloured by chain, side view.
PDB entry 3ry9 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Mechanisms for the evolution of a derived function in the ancestral glucocorticoid receptor.
Carroll SM, Ortlund EA, Thornton JW
PLoS Genet 7 e1002117 (2011)
PMID: 21698144

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ancestral Glucocorticoid Receptor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 28.8 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Retinoid X Receptor

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand 1CA 2 x 1CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 139.421Å b: 49.144Å c: 100.369Å
α: 90° β: 105.82° γ: 90°
R-values:
R R work R free
0.183 0.181 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Emergence and evolution of the renin-angiotensin-aldosterone system.
Fournier et al. (2012)
6 more

3 mentions without citation

Historical contingency and its biophysical basis in glucocorticoid receptor evolution.
Harms et al. (2014)
2 more