› 3rzn

X-ray diffraction
1.1Å resolution

Crystal Structure of Human Glycolipid Transfer Protein complexed with 3-O-sulfo-galactosylceramide containing nervonoyl acyl chain (24:1)

Released:
DOI: 10.2210/pdb3rzn/pdb
PDB entry 3rzn coloured by chain, front view.
PDB entry 3rzn coloured by chain, side view.
PDB entry 3rzn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Enhanced selectivity for sulfatide by engineered human glycolipid transfer protein.
Samygina VR, Popov AN, Cabo-Bilbao A, Ochoa-Lizarralde B, Goni-de-Cerio F, Zhai X, Molotkovsky JG, Patel DJ, Brown RE, Malinina L
Structure 19 1644-54 (2011)
PMID: 22078563

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycolipid transfer protein Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZD2 (Residues: 1-209; Coverage: 100%)
Gene name: GLTP
Sequence domains: Glycolipid transfer protein (GLTP)
Structure domains: Glycolipid transfer protein

Ligands and Environments

1 bound ligand:
Ligand CIS 1 x CIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 74.891Å b: 50.06Å c: 65.404Å
α: 90° β: 118.52° γ: 90°
R-values:
R R work R free
0.139 0.138 0.157
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Sphingolipids and their metabolism in physiology and disease.
Hannun et al. (2018)
5 more

6 mentions without citation

The repertoire of protein-sulfatide interactions reveal distinct modes of sulfatide recognition.
Capelluto DGS. (2022)
5 more