› 3rzv

X-ray diffraction
1.67Å resolution

The Crystal Structure of a E280A Mutant of the Catalytic Domain of AMSH

Released:
DOI: 10.2210/pdb3rzv/pdb
PDB entry 3rzv coloured by chain, front view.
PDB entry 3rzv coloured by chain, side view.
PDB entry 3rzv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and thermodynamic comparison of the catalytic domain of AMSH and AMSH-LP: nearly identical fold but different stability.
Davies CW, Paul LN, Kim MI, Das C
J Mol Biol 413 416-29 (2011)
PMID: 21888914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
STAM-binding protein Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O95630 (Residues: 219-424; Coverage: 49%)
Gene names: AMSH, STAMBP
Sequence domains: JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 53.437Å b: 53.437Å c: 128.828Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.205
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
10 more

7 mentions without citation

Early‑onset epilepsy and microcephaly‑capillary malformation syndrome caused by a novel STAMBP mutation in a Chinese boy.
Wu et al. (2019)
6 more