› 3s0t

X-ray diffraction
1.26Å resolution

Crystal structure of the CofA Type IV pilin subunit from enterotoxigenic E. coli

Released:
DOI: 10.2210/pdb3s0t/pdb
PDB entry 3s0t coloured by chain, front view.
PDB entry 3s0t coloured by chain, side view.
PDB entry 3s0t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural characterization of CFA/III and Longus type IVb pili from enterotoxigenic Escherichia coli.
Kolappan S, Roos J, Yuen AS, Pierce OM, Craig L
J Bacteriol 194 2725-35 (2012)
PMID: 22447901

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CFA/III pilin Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 19.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59393 (Residues: 59-238; Coverage: 76%)
Gene name: cofA
Sequence domains: Toxin-coregulated pilus subunit TcpA
Structure domains: TcpA-like pilin

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P1
Unit cell:
a: 29.5Å b: 34.15Å c: 64.23Å
α: 95.75° β: 97.38° γ: 94°
R-values:
R R work R free
0.142 0.139 0.181
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Type IV pilin proteins: versatile molecular modules.
Giltner et al. (2012)
2 more

5 mentions without citation

Structural characterization of CFA/III and Longus type IVb pili from enterotoxigenic Escherichia coli.
Kolappan et al. (2012)
4 more