› 3s1s

X-ray diffraction
2.35Å resolution

Characterization and crystal structure of the type IIG restriction endonuclease BpuSI

Released:
DOI: 10.2210/pdb3s1s/pdb
Model geometry
Fit model/data
PDB entry 3s1s coloured by chain, front view.
PDB entry 3s1s coloured by chain, side view.
PDB entry 3s1s coloured by chain, top view.
Source organism: Bacillus pumilus
Primary publication:
Characterization and crystal structure of the type IIG restriction endonuclease RM.BpuSI.
Shen BW, Xu D, Chan SH, Zheng Y, Zhu Z, Xu SY, Stoddard BL
OpenAccess logo Nucleic Acids Res 39 8223-36 (2011)
PMID: 21724614

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
site-specific DNA-methyltransferase (adenine-specific) Chain: A
Molecule details ›
Chain: A
Length: 878 amino acids
Theoretical weight: 100.07 KDa
Source organism: Bacillus pumilus
Expression system: Escherichia coli
UniProt:
  • Canonical: G1K3S1 (Residues: 1-878; Coverage: 100%)
Gene name: Bacillus pumilus
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
3 bound ligands:
Ligand EDO 20 x EDO
Ligand MN 1 x MN
Ligand IOD 38 x IOD
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.1, RIGAKU MICROMAX-007 HF, ALS BEAMLINE 5.0.2, ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 213.472Å b: 215.732Å c: 73.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.268
Expression system: Escherichia coli

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Citations

4 review citations

Type I restriction enzymes and their relatives.
Loenen et al. (2014)
3 more

7 mentions without citation

Structure and operation of the DNA-translocating type I DNA restriction enzymes.
Kennaway et al. (2012)
6 more