› 3s2w

X-ray diffraction
2.45Å resolution

The crystal structure of a MarR transcriptional regulator from Methanosarcina mazei Go1

Released:
DOI: 10.2210/pdb3s2w/pdb
PDB entry 3s2w coloured by chain, front view.
PDB entry 3s2w coloured by chain, side view.
PDB entry 3s2w coloured by chain, top view.
Source organism: Methanosarcina mazei Go1
Entry authors: Tan K, Li H, Gu M, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH marR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 159 amino acids
Theoretical weight: 18.8 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8PVU7 (Residues: 1-156; Coverage: 100%)
Gene name: MM_1863
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 14 x SO4
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 42.943Å b: 300.077Å c: 55.711Å
α: 90° β: 111.13° γ: 90°
R-values:
R R work R free
0.183 0.179 0.246
Expression system: Escherichia coli BL21

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