› 3s32

X-ray diffraction
2.45Å resolution

Crystal structure of Ash2L N-terminal domain

Released:
DOI: 10.2210/pdb3s32/pdb
PDB entry 3s32 coloured by chain, front view.
PDB entry 3s32 coloured by chain, side view.
PDB entry 3s32 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the trithorax group protein ASH2L reveals a forkhead-like DNA binding domain.
Sarvan S, Avdic V, Tremblay V, Chaturvedi CP, Zhang P, Lanouette S, Blais A, Brunzelle JS, Brand M, Couture JF
Nat Struct Mol Biol 18 857-9 (2011)
PMID: 21642971

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Set1/Ash2 histone methyltransferase complex subunit ASH2 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBL3 (Residues: 95-280; Coverage: 30%)
Gene names: ASH2L, ASH2L1
Sequence domains: Set1/Ash2 histone methyltransferase complex subunit ASH2-like, WH
Structure domains: DNA primase DNAg catalytic core, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 49.893Å b: 49.893Å c: 167.542Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.307 0.225 0.263
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Trithorax group proteins: switching genes on and keeping them active.
Schuettengruber et al. (2011)
7 more

4 mentions without citation

Crystal structure of the trithorax group protein ASH2L reveals a forkhead-like DNA binding domain.
Sarvan et al. (2011)
3 more