› 3s4l

X-ray diffraction
2.3Å resolution

The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb3s4l/pdb
PDB entry 3s4l coloured by chain, front view.
PDB entry 3s4l coloured by chain, side view.
PDB entry 3s4l coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference.
Beloglazova N, Petit P, Flick R, Brown G, Savchenko A, Yakunin AF
EMBO J 30 4616-27 (2011)
PMID: 22009198

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas3-HD Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 28.45 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57829 (Residues: 1-244; Coverage: 100%)
Gene names: MJ0384, cas3, cas3''
Sequence domains: Cas3, HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 59.073Å b: 59.073Å c: 162.879Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
28 more

5 mentions without citation

Annotation and Classification of CRISPR-Cas Systems.
Makarova et al. (2015)
4 more