› 3s5b

X-ray diffraction
1.8Å resolution

Crystal Structure of CED-3 Protease Suppressor-6 (CPS-6) from Caenorhabditis elegans

Released:
DOI: 10.2210/pdb3s5b/pdb
PDB entry 3s5b coloured by chain, front view.
PDB entry 3s5b coloured by chain, side view.
PDB entry 3s5b coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural insights into apoptotic DNA degradation by CED-3 protease suppressor-6 (CPS-6) from Caenorhabditis elegans.
Lin JL, Nakagawa A, Lin CL, Hsiao YY, Yang WZ, Wang YT, Doudeva LG, Skeen-Gaar RR, Xue D, Yuan HS
J Biol Chem 287 7110-20 (2012)
PMID: 22223640

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease G, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.6 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95NM6 (Residues: 63-303; Coverage: 78%)
Gene names: C41D11.8, cps-6
Sequence domains: DNA/RNA non-specific endonuclease
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 69.221Å b: 45.379Å c: 80.31Å
α: 90° β: 104.17° γ: 90°
R-values:
R R work R free
0.168 0.166 0.21
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Endonucleases and apoptosis in animals.
Aleksandrushkina et al. (2012)
5 other articles

4 mentions without citation

Oxidative Stress Impairs Cell Death by Repressing the Nuclease Activity of Mitochondrial Endonuclease G.
Lin et al. (2016)
3 more