› 3s7q

X-ray diffraction
1.75Å resolution

Crystal Structure of a Monomeric Infrared Fluorescent Deinococcus radiodurans Bacteriophytochrome chromophore binding domain

Released:
DOI: 10.2210/pdb3s7q/pdb
PDB entry 3s7q coloured by chain, front view.
PDB entry 3s7q coloured by chain, side view.
PDB entry 3s7q coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
Structure-guided engineering enhances a phytochrome-based infrared fluorescent protein.
Auldridge ME, Satyshur KA, Anstrom DM, Forest KT
J Biol Chem 287 7000-9 (2012)
PMID: 22210774

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 37.21 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-321; Coverage: 43%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand LBV 1 x LBV
Ligand LBW 1 x LBW
Ligand GOL 2 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 95.129Å b: 55.072Å c: 69.969Å
α: 90° β: 92.23° γ: 90°
R-values:
R R work R free
0.189 0.187 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.
Rodriguez et al. (2017)
13 more

4 mentions without citation

Phytochromes in Agrobacterium fabrum.
Lamparter et al. (2021)
3 more