› 3s8j

X-ray diffraction
2.6Å resolution

Crystal structure of a papaya latex serine protease inhibitor (PPI) at 2.6A resolution

Released:
DOI: 10.2210/pdb3s8j/pdb
PDB entry 3s8j coloured by chain, front view.
PDB entry 3s8j coloured by chain, side view.
PDB entry 3s8j coloured by chain, top view.
Source organism: Carica papaya
Primary publication:
The plasticity of the β-trefoil fold constitutes an evolutionary platform for protease inhibition.
Azarkan M, Martinez-Rodriguez S, Buts L, Baeyens-Volant D, Garcia-Pino A
J Biol Chem 286 43726-43734 (2011)
PMID: 22027836

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Latex serine proteinase inhibitor Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.68 KDa
Source organism: Carica papaya
UniProt:
  • Canonical: P80691 (Residues: 1-184; Coverage: 100%)
Sequence domains: Trypsin and protease inhibitor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand ACT 5 x ACT
Ligand GOL 1 x GOL
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P31
Unit cell:
a: 74.699Å b: 74.699Å c: 78.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.15 0.213

Quick links

Citations

2 review citations

Juggling jobs: roles and mechanisms of multifunctional protease inhibitors in plants.
Grosse-Holz et al. (2016)
1 more

2 mentions without citation

The plasticity of the β-trefoil fold constitutes an evolutionary platform for protease inhibition.
Azarkan et al. (2011)
1 more