› 3sb7

X-ray diffraction
2.7Å resolution

Cu-mediated Trimer of T4 Lysozyme D61H/K65H/R76H/R80H by Synthetic Symmetrization

Released:
DOI: 10.2210/pdb3sb7/pdb
Model geometry
Fit model/data
PDB entry 3sb7 coloured by chain, front view.
PDB entry 3sb7 coloured by chain, side view.
PDB entry 3sb7 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
An approach to crystallizing proteins by metal-mediated synthetic symmetrization.
Laganowsky A, Zhao M, Soriaga AB, Sawaya MR, Cascio D, Yeates TO
Protein Sci 20 1876-90 (2011)
PMID: 21898649

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 18.61 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-162; Coverage: 99%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: R3
Unit cell:
a: 119.91Å b: 119.91Å c: 64.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.187 0.226
Expression system: Escherichia coli

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Citations

9 review citations

The design of symmetric protein nanomaterials comes of age in theory and practice.
Yeates et al. (2016)
8 more