› 3sch

X-ray diffraction
2.1Å resolution

Co(II)-HppE with R-HPP

Released:
DOI: 10.2210/pdb3sch/pdb
PDB entry 3sch coloured by chain, front view.
PDB entry 3sch coloured by chain, side view.
PDB entry 3sch coloured by chain, top view.
Source organism: Streptomyces wedmorensis
Primary publication:
Structural basis of regiospecificity of a mononuclear iron enzyme in antibiotic fosfomycin biosynthesis.
Yun D, Dey M, Higgins LJ, Yan F, Liu HW, Drennan CL
J Am Chem Soc 133 11262-9 (2011)
PMID: 21682308

Function and Biology Details

Reaction catalysed: 
(S)-2-hydroxypropylphosphonate + H(2)O(2) = (1R,2S)-1,2-epoxypropylphosphonate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-hydroxypropylphosphonic acid epoxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 21.6 KDa
Source organism: Streptomyces wedmorensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56185 (Residues: 1-198; Coverage: 100%)
Gene names: fom4, hppE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TB6 2 x TB6
Ligand CO 2 x CO
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P6522
Unit cell:
a: 86.12Å b: 86.12Å c: 219.056Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.217 0.245
Expression system: Escherichia coli

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Citations

5 review citations

Enzymatic chemistry of cyclopropane, epoxide, and aziridine biosynthesis.
Thibodeaux et al. (2012)
4 more