› 3sd9

X-ray diffraction
1.83Å resolution

Crystal structure of serratia fonticola SFH-I: Source of the nucleophile in the catalytic mechanism of mono-zinc metallo-beta-lactamases

Released:
DOI: 10.2210/pdb3sd9/pdb
Model geometry
Fit model/data
PDB entry 3sd9 coloured by chain, front view.
PDB entry 3sd9 coloured by chain, side view.
PDB entry 3sd9 coloured by chain, top view.
Source organism: Serratia fonticola
Entry authors: Fonseca F, Saavedra MJ, Correia A, Spencer J

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.29 KDa
Source organism: Serratia fonticola
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RMI1 (Residues: 3-234; Coverage: 99%)
Gene name: sfhI
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 32.965Å b: 86.412Å c: 72.193Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.185 0.183 0.213
Expression system: Escherichia coli

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