› 3sdm

X-ray diffraction
6.6Å resolution

Structure of oligomeric kinase/RNase Ire1 in complex with an oligonucleotide

Released:
DOI: 10.2210/pdb3sdm/pdb
PDB entry 3sdm coloured by chain, front view.
PDB entry 3sdm coloured by chain, side view.
PDB entry 3sdm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cofactor-mediated conformational control in the bifunctional kinase/RNase Ire1.
Korennykh AV, Egea PF, Korostelev AA, Finer-Moore J, Stroud RM, Zhang C, Shokat KM, Walter P
BMC Biol 9 48 (2011)
PMID: 21729334

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetradecamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase/endoribonuclease IRE1 Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 448 amino acids
Theoretical weight: 51.88 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32361 (Residues: 641-1115; Coverage: 41%)
Gene names: ERN1, IRE1, YHR079C
Sequence domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand SEP 14 x SEP
Ligand TPO 7 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C222
Unit cell:
a: 91.65Å b: 580.67Å c: 177.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.288 0.286 0.317
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The unfolded protein response: from stress pathway to homeostatic regulation.
Walter et al. (2011)
18 more

5 mentions without citation

Cofactor-mediated conformational control in the bifunctional kinase/RNase Ire1.
Korennykh et al. (2011)
4 more