› 3sei

X-ray diffraction
2.4Å resolution

Crystal Structure of Caskin1 Tandem SAMs

Released:
DOI: 10.2210/pdb3sei/pdb
PDB entry 3sei coloured by chain, front view.
PDB entry 3sei coloured by chain, side view.
PDB entry 3sei coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK.
Stafford RL, Hinde E, Knight MJ, Pennella MA, Ear J, Digman MA, Gratton E, Bowie JU
Structure 19 1826-36 (2011)
PMID: 22153505

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caskin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WXD9 (Residues: 470-605; Coverage: 10%)
Gene names: CASKIN1, KIAA1306
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41
Unit cell:
a: 93.114Å b: 93.114Å c: 39.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Hunting for Novel Routes in Anticancer Drug Discovery: Peptides against Sam-Sam Interactions.
Mercurio et al. (2022)
1 more

6 mentions without citation

Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK.
Stafford et al. (2011)
5 more