› 3sfu

X-ray diffraction
2.5Å resolution

crystal structure of murine norovirus RNA dependent RNA polymerase in complex with ribavirin

Released:
DOI: 10.2210/pdb3sfu/pdb
PDB entry 3sfu coloured by chain, front view.
PDB entry 3sfu coloured by chain, side view.
PDB entry 3sfu coloured by chain, top view.
Source organism: Murine norovirus 1
Primary publication:
Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin.
Alam I, Lee JH, Cho KJ, Han KR, Yang JM, Chung MS, Kim KH
Virology 426 143-51 (2012)
PMID: 22341781

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 517 amino acids
Theoretical weight: 58.52 KDa
Source organism: Murine norovirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80J95 (Residues: 1181-1686; Coverage: 30%)
Gene name: ORF1
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand RBV 3 x RBV
Ligand SO4 27 x SO4
Ligand MG 3 x MG
Ligand GOL 26 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2
Unit cell:
a: 119.952Å b: 196.084Å c: 109.206Å
α: 90° β: 114.12° γ: 90°
R-values:
R R work R free
0.182 0.181 0.229
Expression system: Escherichia coli

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Citations

7 review citations

Treatment of norovirus infections: moving antivirals from the bench to the bedside.
Kaufman et al. (2014)
6 more

1 mention without citation

Potential molecular targets of nonstructural proteins for the development of antiviral drugs against SARS-CoV-2 infection.
Liu et al. (2021)