› 3sfx

X-ray diffraction
2Å resolution

Cryptococcus neoformans protein farnesyltransferase in complex with FPT-II and tipifarnib

Released:
DOI: 10.2210/pdb3sfx/pdb
PDB entry 3sfx coloured by chain, front view.
PDB entry 3sfx coloured by chain, side view.
PDB entry 3sfx coloured by chain, top view.
Source organism: Cryptococcus neoformans var. grubii H99
Primary publication:
Structures of Cryptococcus neoformans protein farnesyltransferase reveal strategies for developing inhibitors that target fungal pathogens.
Hast MA, Nichols CB, Armstrong SM, Kelly SM, Hellinga HW, Alspaugh JA, Beese LS
J Biol Chem 286 35149-62 (2011)
PMID: 21816822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241341 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cryptococcus neoformans protein farnesyltransferase alpha subunit Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.93 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli
Structure domains: Protein prenylyltransferase
Cryptococcus neoformans protein farnesyltransferase beta subunit Chain: B
Molecule details ›
Chain: B
Length: 520 amino acids
Theoretical weight: 56.81 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli
Structure domains: Glycosyltransferase

Ligands and Environments

5 bound ligands:
Ligand ZN 1 x ZN
Ligand 3FX 3 x 3FX
Ligand FII 1 x FII
Ligand JAN 1 x JAN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P43212
Unit cell:
a: 142.176Å b: 142.176Å c: 130.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.199
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The antifungal pipeline: a reality check.
Perfect JR. (2017)
11 more

3 mentions without citation

Structure-guided approaches to targeting stress responses in human fungal pathogens.
LeBlanc et al. (2020)
2 more