› 3sgk

X-ray diffraction
2.4Å resolution

Unique carbohydrate/carbohydrate interactions are required for high affinity binding of FcgIII and antibodies lacking core fucose

Released:
DOI: 10.2210/pdb3sgk/pdb
PDB entry 3sgk coloured by chain, front view.
PDB entry 3sgk coloured by chain, side view.
PDB entry 3sgk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgammaRIII and antibodies lacking core fucose.
Ferrara C, Grau S, Jäger C, Sondermann P, Brünker P, Waldhauer I, Hennig M, Ruf A, Rufer AC, Stihle M, Umaña P, Benz J
Proc Natl Acad Sci U S A 108 12669-74 (2011)
PMID: 21768335

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134854 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.34 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 106-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor III-A Chain: C
Molecule details ›
Chain: C
Length: 204 amino acids
Theoretical weight: 23.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08637 (Residues: 19-208; Coverage: 80%)
Gene names: CD16A, FCG3, FCGR3, FCGR3A, IGFR3
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 67.343Å b: 88.227Å c: 141.133Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.25
Expression systems:
  • Cricetulus griseus
  • Homo sapiens

Quick links

Citations

141 review citations

IgG subclasses and allotypes: from structure to effector functions.
Vidarsson et al. (2014)
140 more

26 mentions without citation

Function and 3D structure of the N-glycans on glycoproteins.
Nagae et al. (2012)
25 more