› 3sik

X-ray diffraction
2.15Å resolution

Crystal structure of the Bacillus anthracis hemophore IsdX1 complexed with heme

Released:
DOI: 10.2210/pdb3sik/pdb
PDB entry 3sik coloured by chain, front view.
PDB entry 3sik coloured by chain, side view.
PDB entry 3sik coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Differential function of lip residues in the mechanism and biology of an anthrax hemophore.
Ekworomadu MT, Poor CB, Owens CP, Balderas MA, Fabian M, Olson JS, Murphy F, Bakkalbasi E, Balkabasi E, Honsa ES, He C, Goulding CW, Maresso AW
PLoS Pathog 8 e1002559 (2012)
PMID: 22412371

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NEAT domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.85 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L8PY92 (Residues: 27-152; Coverage: 97%)
Gene name: GBAA_4788
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43
Unit cell:
a: 64.978Å b: 64.978Å c: 74.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
3 more

4 mentions without citation

Differential function of lip residues in the mechanism and biology of an anthrax hemophore.
Ekworomadu et al. (2012)
3 more