› 3sjs

X-ray diffraction
1.9Å resolution

Crystal structure of URE3-binding protein from Entamoeba histolytica, (D127A,N129A) mutant, native form

Released:
DOI: 10.2210/pdb3sjs/pdb
PDB entry 3sjs coloured by chain, front view.
PDB entry 3sjs coloured by chain, side view.
PDB entry 3sjs coloured by chain, top view.
Source organism: Entamoeba histolytica
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EF-hand calcium-binding domain containing protein Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 25.68 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4MBE2 (Residues: 8-227; Coverage: 97%)
Gene name: EHI_060740
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2221
Unit cell:
a: 46.25Å b: 68.29Å c: 130.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.221
Expression system: Escherichia coli

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