› 3sk9

X-ray diffraction
1.8Å resolution

Crystal structure of the Thermus thermophilus cas3 HD domain

Released:
DOI: 10.2210/pdb3sk9/pdb
PDB entry 3sk9 coloured by chain, front view.
PDB entry 3sk9 coloured by chain, side view.
PDB entry 3sk9 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural and biochemical analysis of nuclease domain of clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 3 (Cas3).
Mulepati S, Bailey S
J Biol Chem 286 31896-903 (2011)
PMID: 21775431

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176135 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease/helicase Cas3 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.72 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53VY2 (Residues: 6-260; Coverage: 28%)
Gene names: TTHB187, cas3
Sequence domains: Cas3, HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P4322
Unit cell:
a: 48.638Å b: 48.638Å c: 206.011Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.195
Expression system: Escherichia coli

Quick links

Citations

32 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
31 more

5 mentions without citation

Structural insights into the activation of metabotropic glutamate receptors.
Koehl et al. (2019)
4 more