› 3skj

X-ray diffraction
2.5Å resolution

Structural And Functional Characterization of an Agonistic Anti-Human EphA2 Monoclonal Antibody

Released:
DOI: 10.2210/pdb3skj/pdb
PDB entry 3skj coloured by chain, front view.
PDB entry 3skj coloured by chain, side view.
PDB entry 3skj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody.
Peng L, Oganesyan V, Damschroder MM, Wu H, Dall'Acqua WF
J. Mol. Biol. 413 390-405 (2011)
PMID: 21867711

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210990 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody, light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 214 amino acids
Theoretical weight: 23.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody, heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 231 amino acids
Theoretical weight: 24.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Ephrin type-A receptor 2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 206 amino acids
Theoretical weight: 23.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29317 (Residues: 23-202; Coverage: 19%)
Gene names: ECK, EPHA2
Sequence domains: Ephrin receptor ligand binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 78.94Å b: 120.859Å c: 286.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.271
Expression system: Homo sapiens

Quick links

Citations

6 review citations

Therapeutic targeting of EPH receptors and their ligands.
Boyd et al. (2014)
5 more

2 mentions without citation

Recurrent Potent Human Neutralizing Antibodies to Zika Virus in Brazil and Mexico.
Robbiani et al. (2017)
1 more