› 3skz

X-ray diffraction
2.6Å resolution

Crystal structure of the 2'- deoxyguanosine riboswitch bound to guanosine

Released:
DOI: 10.2210/pdb3skz/pdb
PDB entry 3skz coloured by chain, front view.
PDB entry 3skz coloured by chain, side view.
PDB entry 3skz coloured by chain, top view.
Source organism: Mesoplasma florum
Primary publication:
Structural principles of nucleoside selectivity in a 2'-deoxyguanosine riboswitch.
Pikovskaya O, Polonskaia A, Patel DJ, Serganov A
Nat Chem Biol 7 748-55 (2011)
PMID: 21841796

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196737 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (68-MER) Chains: A, B
Molecule details ›
Chains: A, B
Length: 68 nucleotides
Theoretical weight: 22.02 KDa
Source organism: Mesoplasma florum
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand GMP 2 x GMP
Ligand MG 3 x MG
Ligand SO4 8 x SO4
Ligand SIN 1 x SIN
2 modified residues:
Ligand GTP 2 x GTP
Ligand CCC 2 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 34.806Å b: 47.208Å c: 227.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.275
Expression system: Not provided

Quick links

Citations

6 review citations

A decade of riboswitches.
Serganov et al. (2013)
5 more

6 mentions without citation

Molecular recognition and function of riboswitches.
Serganov et al. (2012)
5 more