› 3slk

X-ray diffraction
3Å resolution

Structure of ketoreductase and enoylreductase didomain from modular polyketide synthase

Released:
DOI: 10.2210/pdb3slk/pdb
PDB entry 3slk coloured by chain, front view.
PDB entry 3slk coloured by chain, side view.
PDB entry 3slk coloured by chain, top view.
Source organism: Saccharopolyspora spinosa
Primary publication:
Divergence of multimodular polyketide synthases revealed by a didomain structure.
Zheng J, Gay DC, Demeler B, White MA, Keatinge-Clay AT
Nat Chem Biol 8 615-21 (2012)
PMID: 22634636

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase extender module 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 795 amino acids
Theoretical weight: 83.42 KDa
Source organism: Saccharopolyspora spinosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ALM5 (Residues: 1216-1989; Coverage: 36%)
Gene name: spnB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NDP 4 x NDP
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1, ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 73.104Å b: 110.195Å c: 202.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The structures of type I polyketide synthases.
Keatinge-Clay AT. (2012)
13 more

11 mentions without citation

Stereocontrol within polyketide assembly lines.
Keatinge-Clay AT. (2016)
10 more