› 3sqf

X-ray diffraction
1.63Å resolution

Crystal structure of monomeric M-PMV retroviral protease

Released:
DOI: 10.2210/pdb3sqf/pdb
PDB entry 3sqf coloured by chain, front view.
PDB entry 3sqf coloured by chain, side view.
PDB entry 3sqf coloured by chain, top view.
Source organism: Mason-Pfizer monkey virus
Primary publication:
Crystal structure of a monomeric retroviral protease solved by protein folding game players.
Khatib F, DiMaio F, Cooper S, Kazmierczyk M, Gilski M, Krzywda S, Zabranska H, Pichova I, Thompson J, Popović Z, Jaskolski M, Baker D
Nat Struct Mol Biol 18 1175-7 (2011)
PMID: 21926992

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease 13 kDa Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.9 KDa
Source organism: Mason-Pfizer monkey virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07570 (Residues: 760-873; Coverage: 13%)
Gene name: gag-pro
Sequence domains: Retroviral aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 26.76Å b: 86.62Å c: 39.31Å
α: 90° β: 104.6° γ: 90°
R-values:
R R work R free
0.172 0.169 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Computational methods in drug discovery.
Sliwoski et al. (2014)
28 more

4 mentions without citation

High-resolution structure of a retroviral protease folded as a monomer.
Gilski et al. (2011)
3 more